DPlex Express
High-throughput automated 3D fluorescence imaging across 96 or 384 wells.
Emulsion digital PCR (edPCR)
AI-scaled
digital PCR
Bringing digital PCR precision to high-throughput molecular testing.
DPlexBio’s emulsion digital PCR (edPCR) combines simple emulsion generation, 3D fluorescence imaging, and AI-powered analysis to enable absolute nucleic acid quantitation without complex and expensive microfluidic partitioning.
The paradigm shift
Shifting the scaling problem from chips to compute.
Digital PCR provides absolute nucleic acid quantitation with exceptional precision. Conventional systems achieve it through complex and expensive microfluidics. DPlexBio takes a simpler path.
Conventional digital PCR
Precision is built into the consumable.
Microfluidic chips create highly uniform partitions before PCR.
Scaling depends on precision-manufactured physical components.
DPlexBio edPCR
Precision is built into the measurement.
Simple shaking creates variable-volume droplets that are measured by 3D imaging and AI analysis.
Scaling shifts toward imaging, software and computation.
edPCR replaces microfluidic partitioning with 3D imaging and computation.
Simple workflow
Digital PCR, simplified.
Generate droplets directly in standard-format wells, thermocycle, image in 3D, and let computational analysis determine absolute target concentration.
See edPCR in action
Digital PCR, simplified
From a quick shake to absolute nucleic acid quantitation. Explore how DPlexBio combines emulsion generation, 3D fluorescence imaging, and computational analysis in one streamlined workflow.
A different approach
Different-sized droplets are not a limitation. Polydispersity is an advantage.
Conventional digital PCR has been built around the assumption that accurate quantitation requires uniform partitions. We have demonstrated that accurate droplet digital PCR does not require monodisperse droplets—and, in fact, benefits from polydisperse droplets — and thus does not require an expensive instrument or microfluidic chip to generate them.
Simple droplets. Sophisticated measurement.
Computational measurement
From fluorescence images to molecular counts.
DPlexBio’s emulsion digital PCR (edPCR) software identifies individual droplets in 3D, measures their volumes and fluorescence, classifies PCR-positive reactions, and calculates absolute nucleic acid concentration.
AI-scaled digital PCR Physics-informed and generative AI for faster 3D imaging.
Why edPCR
Digital PCR performance. qPCR-like workflow.
Commercial platform
Commercial platform.
One integrated edPCR platform.
DPlex Pulse
Programmable shaker for rapid emulsion generation.
Plates & Reagents
Purpose-built edPCR consumables that offer high flexibility – single reaction, strip of 8 reactions, full 96 or 384 well plates.
DPlex Inspect Software
Turn-key edPCR analysis based on AI-enhanced 3D image processing.
Company
Making digital PCR practical at scale.
DPlexBio is a Life Sciences company commercializing emulsion digital PCR technology developed from research at the University of Washington. Our goal is to make digital PCR precision practical and affordable for routine research and high-throughput molecular testing.
- Scientific foundationDroplet microfluidics and digital PCR research
- Platform developmentAutomated edPCR instrumentation, software and consumables
- External validationBeta tested in a CLIA laboratory
Scale molecular measurements with computation.
DPlexBio is developing AI-scaled digital PCR for research, clinical laboratories, and high-throughput molecular diagnostic applications.